In silico kinetic model of Chlamydomonas reinhardtii

Mariajose Castellanos, Chemical and Biochemical Engineering
Goncalo Maia, Chemical and Biochemical Engineering


A kinetic metabolic model of the microalgae Chlamydomonas reinhardtii encompassing 15 pathways, 320 reactions, and 218 compounds was created. A total of 376 Km enzymatic kinetic constants (63% of total) as
well as 182 turnover numbers (84% of total) were determined by using the BRENDA database for enzyme properties. The model predicts previously published experimental observations, including the augmentation in lipid production and decrease in biomass generation under nitrogen-deficient conditions and the effect of up-regulating Acetyl-Coa Carboxylase (EC: (previously documented during the Aquatic Species Program (ASP)).